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Catalog Number | orb1257699 |
---|---|
Category | Antibodies |
Description | CRTAP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human CRTAP (NP_006362.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 47kDa |
Target | CRTAP |
UniProt ID | O75718 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Cartilage-associated protein, CRTAP, CASP Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of SW480 cells, using CRTAP antibody (orb1257699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using CRTAP antibody (orb1257699) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using CRTAP antibody (orb1257699) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CRTAP antibody (orb1257699) at dilution of 1:100 (40x lens).
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