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Catalog Number | orb1262243 |
---|---|
Category | Antibodies |
Description | CROT Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit Ig |
Immunogen | This CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human CROT. |
Concentration | batch dependent |
Dilution range | For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 70 kDa |
Target | CROT |
UniProt ID | Q9UKG9 |
NCBI | Q9UKG9 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Peroxisomal carnitine O-octanoyltransferase, COT, Read more... |
Note | For research use only |
Application notes | For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000 |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of CROT Antibody in K562 cell line and mouse liver tissue lysates (35 ug/lane)
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