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Catalog Number | orb1249260 |
---|---|
Category | Antibodies |
Description | Crot Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Rat |
Immunogen | The immunogen for this antibody is: RSCLETDAEKLVE |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in Rat Kidney lysates (calculated MW of 70.3kDa according to NP_114193.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Liver. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | Crot |
UniProt ID | P11466 |
NCBI | NP_114193.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | Crot, carnitine O-octanoyltransferase, COT, peroxi Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in Rat Kidney lysates (calculated MW of 70.3kDa according to NP_114193.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Liver. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1249260 (0.3 ug/ml) staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1249260 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1249260 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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