CRMP-5 antibody

• Catalog Number: orb1473693
• Category: Antibodies
• Description: Mouse monoclonal antibody to CRMP-5
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human CRMP-5. The exact sequence is proprietary.
• Dilution range: WB (1/1000 - 1/2000), IF/IC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: DPYSL5
• Entrez: 56896, 100910964, 65254, 65208
• UniProt ID: Q9JHU0, Q9BPU6, Q9EQF6
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: CRMP5; ULIP6; Dihydropyrimidinase-related protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CRMP-5 expression in SHSY5Y (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 61 kD)

Immunofluorescent analysis of CRMP-5 staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).