CRBN Antibody

• Catalog Number: orb1239959
• Category: Antibodies
• Description: CRBN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN.The immunogen is located within the last 50 amino acids of CRBN.
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 49 kDa Observed: 54 kDa
• Target: CRBN
• UniProt ID: Q96SW2
• NCBI: NP_057386
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: CRBN antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Cereblon, AD-006, DKFZp781K0715, MGC27358, MRT2A
• Note: For research use only
• Application notes: CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Validation of CRBN in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with orb1239959 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

WB Validation in Human Cells. Loading: 15 µg of cell lysate Antibodies: CRBN orb1239959, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Human Testis. Loading: 15 µg of human testis lysate Antibodies: CRBN orb1239959, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A: 0.5 µg/mL Lane B: 1 µg/mL.

WB Validation in Mouse Tissues. Loading: 15 µg of mouse tissue lysate Antibodies: CRBN orb1239959, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Tissues. Loading: 15 µg of rat tissue lysate Antibodies: CRBN orb1239959, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.