CR1 Antibody

• Catalog Number: orb1671822
• Category: Antibodies
• Description: CR1 Antibody
• Clonality: Recombinant
• Clone Number: HB-8592
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Animals were immunized with purified CR1 from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CR1
• UniProt ID: P17927
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CR1, C3BR, C4BR, HB8592, HB 8592
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of Jurkat cells using anti-CD35 antibody (HB-8592). Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of HB-8592 (orb1671822) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671822- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using the anti-CD35 antibody HB-8592. Jurkat cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r1 version of HB-8592 (orb1671822 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.