COX4I1 Rabbit Polyclonal Antibody

• Catalog Number: orb182884
• Category: Antibodies
• Description: COX4I1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human COX4I1 (51-169/169aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17 kDa
• Target: COX4I1
• UniProt ID: P13073
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: COX41_HUMAN; Cytochrome c oxidase subunit 4 isofor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (COX4) Polyclonal Antibody, Unconjugated (orb182884) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Heart tissue lysates, Lane 3: Mouse Muscle tissue lysates, Lane 4: Rat Kidney tissue lysates, Lane 5: Rat Heart tissue lysates, Lane 6: Rat Muscle tissue lysates, Lane 7: Human HeLa cell lysates, Lane 8: Human Jurkat cell lysates, Lane 9: Human THP-1 cell lysates, Lane 10: Human Raji cell lysates, Lane 11: Human HepG2 cell lysates, Lane 12: Human MCF-7 cell lysates, Primary: Anti-COX4I1 (orb182884) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kDa, Observed band size: 16 kDa.

Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-COX4 Polyclonal Antibody, Unconjugated (orb182884) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.