CoV-2 S1 (319-541) Protein (Biotin)

• Catalog Number: orb753214
• Category: Tools
• Description: The HEK293 derived Biotinylated recombinant protein contains the Coronavirus 2019 Spike Glycoprotein S1 Receptor Binding Domain [ RBD ], Wuhan-Hu-1 strain, amino acids 319-541 fused to His tag & AVI tag at C-terminal and having a molecular mass of 28.7kDa.
• Form/Appearance: Lyophilized freezed dried powder.
• Purity: Protein is > 90% pure as determined SDS-PAGE.
• Solubility (25°C): It is recommended to reconstitute the lyophilized CoV-2 S1 protein in sterile 18M-cm H2O at a concentration of 0.2mg/ml and not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.
• Source: HEK293 Cells
• Storage: Stability: Lyophilized Cov-2 S1 Glycoprotein RBD although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CoV2 Spike protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles
• Buffer/Preservatives: CoV-2 S1 RBD protein is lyophilized from 1x PBS pH-7.4 + 10% trehalose.
• Note: For research use only
• Application notes: Viral Antigens- Sars Proteins
• Expiration Date: 12 months from date of receipt.