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Catalog Number | orb1247655 |
---|---|
Category | Antibodies |
Description | CORO1A Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine, Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-KRLDRLEETVQAK |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human Lymphoblastoid and Human T cell line MOLT4 lysates (calculated MW of 51.0kDa according to NP_009005.1). In transfected HEK293 transiently expressing CORO1A band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Small Intestine. Recommended concentration: 3.75ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CORO1A |
UniProt ID | P31146 |
NCBI | NP_009005.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | CORO1A, Coronin 1, TACO, p57, CLABP, HCORO1, CLIPI Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human Lymphoblastoid and Human T cell line MOLT4 lysates (calculated MW of 51.0kDa according to NP_009005.1). In transfected HEK293 transiently expressing CORO1A band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Small Intestine. Recommended concentration: 3.75ug/ml. |
Expiration Date | 12 months from date of receipt. |
HEK293 overexpressing CORO1A and probed with orb1247655 (mock transfection in first lane).
orb1247655 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247655 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247655 (0.3 ug/ml) staining of Human Lymphoblastoid (RIPA buffer, (35 ug protein in RIPA buffer). Detected by chemiluminescence.
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Porcine, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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