Collagen VII antibody / COL7A1

• Catalog Number: orb639595
• Category: Antibodies
• Description: Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LH7.2
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant full-length human Collagen VII protein was used as the immunogen for this Collagen VII antibody.
• Dilution range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Collagen VII antibody is available for research use only.
• UniProt ID: Q02388
• Storage: Store the Collagen VII antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells with Collagen VII antibody (clone LH7.2, green) and Reddot nuclear stain (red).

IHC staining of FFPE human skin with Collagen VII antibody (clone LH7.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human skin with Collagen VII antibody (clone LH7.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human HeLa cells with Collagen VII antibody (clone LH7.2); Red=isotype control, Blue= LH7.2 antibody.

SDS-PAGE analysis of purified, BSA-free Collagen VII antibody (clone LH7.2) as confirmation of integrity and purity.