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Catalog Number | orb304041 |
---|---|
Category | Antibodies |
Description | Collagen III antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P |
Reactivity | Bovine, Canine, Gallus, Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human Collagen III. Please contact us for the exact immunogen sequence. The peptide is available as orb374884. |
Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
Dilution range | IHC-P: 1:100-500 |
Form/Appearance | 10 mM PBS, 0.02% sodium azide |
Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
Conjugation | Unconjugated |
Target | Collagen III |
Entrez | 1281 |
UniProt ID | P08121, P13941, P02461 |
NCBI | 003656, 775628 |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Alternative names | anti COL3A1 antibody, anti Collagen alpha 1(III) c Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of paraffin embedded rat pancreas tissue using Collagen III antibody (2.5 ug/ml)
IHC-P image of human thyroid cancer tissue using Collagen III antibody (2.5 ug/ml)
IHC-P staining of rat pancreas tissue using Collagen III antibody (2.5 ug/ml)
Immunohistochemical staining of human thyroid cancer tissue using anti-Collagen III (2.5 ug/ml)
Immunohistochemical staining of human endometrial cancer tissue using anti-Collagen III (2.5 ug/ml)
IHC-P staining of human endometrial cancer tissue using anti-Collagen III (2.5 ug/ml)
DOT, ELISA, IHC, IP, WB | |
Bovine, Human, Porcine | |
Rabbit | |
Polyclonal | |
Unconjugated |
DOT, ELISA, IHC, IP, WB | |
Bovine, Human, Porcine | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Bovine, Gallus, Mouse, Rat | |
Canine, Human, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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Useful Collagen III Antibody from Biorbyt. Alkaline antigen retrieval (pH 9.0) caused more background staining. I used citrate pH 6.0 to reduce background staining. I increased the BSA that I normally use from 1% to 3%. There was a nice response. I can say that I got a better response than I expected in my trying. I use these types of collagen antibodies in wound healing and scar studies. Also, It can be used for the state of inflammatory processes of any inflammation or tumor stroma. It does a light nonspecific staining.
- Mehmet Fatih Bozkurt/Afyon Kocatepe University
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