Collagen II Rabbit Polyclonal Antibody

• Catalog Number: orb221349
• Category: Antibodies
• Description: Collagen II Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Guinea pig, Mouse, Porcine, Rat
• Reactivity: Human, Rabbit
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen II (1201-1300/1487aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 117 kDa
• Target: COL2A1
• UniProt ID: P02458
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Collagen II alpha 1; COL2A1; COL2A1 protein; colla
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Fu, Na et al. PCL-PEG-PCL film promotes cartilage regeneration in vivo Cell Prolif, 49, 729-739 (2016)

PubMed: 27647680

Sample: 293T (human) cell Lysate at 40 ug, A549 (human) cell Lysate at 40 ug, MCF-7 (human) cell Lysate at 40 ug, HepG2 (human) cell Lysate at 40 ug, Placenta (mouse) Lysate at 40 ug, MG63 (human) cell Lysate at 40 ug, Primary: Anti-Collagen II (orb221349) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 120 kD.