Collagen II Rabbit Polyclonal Antibody

• Catalog Number: orb182930
• Category: Antibodies
• Description: Collagen II Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen II (231-330/1487aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 117 kDa
• Target: COL2A1
• UniProt ID: P02458
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Collagen II alpha 1; COL2A1; COL2A1 protein; colla
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hong Xue‡ ab, Hongyu Zhou‡ ab, Qiliang Lou‡ ab, Putao Yuan ab, Zhenhua Feng ab, Li Qiao ab, Jiateng Zhang ab, Hongwei Xie ab, Yang Shen ab, Qingliang Ma ab, Shiyu Wang ab, Boya Zhang c, Huali Ye ab, Jiao Cheng ab, Xuewu Sun ORCID logo*ab and Peihua Shi Urolithin B reduces cartilage degeneration and alleviates osteoarthritis by inhibiting inflammation Food & Function, (2024)

PubMed:

Paraformaldehyde-fixed, paraffin embedded (mouse ear), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (orb182930) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, LOVO (Human) Cell Lysate at 40 ug, MCF-7 (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen II (orb182930) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 117 kD.

Sample: bone (Mouse) Lysate at 40 ug, Primary: Anti-Collagen II (orb182930) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 134 kD.

Sample: MG63 (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen II (orb182930) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 117 kD.

Tissue/Cell: Mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (orb182930) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.