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Catalog Number | orb371673 |
---|---|
Category | Antibodies |
Description | Collagen I/COL1A1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1185-1207aa YDFSFLPQPPQEKSQDGGRYYRA), different from the related human sequence by three amino acids, and identical to the related rat sequence. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 138032 MW |
UniProt ID | P11087 |
Sensitivity | > 5000 cells |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Collagen alpha-1 (I) chain;Alpha-1 type I collagen Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of COL1A1 using anti-COL1A1 antibody.Lane 1:mouse NIH/3T3 cell.
IHC analysis of COL1A1 using anti COL1A1 antibody. COL1A1 was detected in a paraffin-embedded section of mouse lung tissue.
IHC analysis of COL1A1 using anti COL1A1 antibody. COL1A1 was detected in a paraffin-embedded section of rat lung tissue.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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