Collagen I Rabbit Polyclonal Antibody

• Catalog Number: orb312178
• Category: Antibodies
• Description: Collagen I Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Sheep
• Reactivity: Human, Mouse, Rabbit, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide (1321-1400/1464aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 130 kDa
• Target: COL1A1
• UniProt ID: P02452
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Collagen type I; Alpha 1 type I collagen; Alpha 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Syverud, Brian C. et al. Quantitative, Label-Free Evaluation of Tissue-Engineered Skeletal Muscle Through Multiphoton Microscopy Tissue Eng Part C Methods, 23, 616-626 (2017)

PubMed: 28810820

Applications

IF

Reactivity

Rat

Conceição, Mariana et al. Histological, Immunohistochemical and Antioxidant Analysis of Skin Wound Healing Influenced by the Topical Application of Brazilian Red Propolis Antioxidants (Basel), 11, 2188 (2022)

PubMed: 36358560

Applications

IHC

Reactivity

Rat

Guo, Fangming et al. Shenfu Administration Improves Cardiac Fibrosis in Rats With Myocardial Ischemia-Reperfusion Through Adenosine A2a Receptor Activation Hum Exp Toxicol, 41, (2022)

PubMed: 35196174

Applications

IHC, WB

Reactivity

Rat

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Collagen I antibody (orb312178), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Polyclonal Antibody, Unconjugated (orb312178) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Polyclonal Antibody, Unconjugated (orb312178) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen I (orb312178) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130, 110 kD, Observed band size: 130 kD.

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug, Lane 2: U-2OS (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen I (orb312178) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD.

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug, Lane 2: U-2OS (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen I (orb312178) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130, 110 kD, Observed band size: 130 kD.

Sample: Ovary (Mouse) Lysate at 40 ug, NIH/3T3 (Mouse) Cell Lysate at 40 ug, Liver (Rat) Lysate at 40 ug, 293T (huamn) Cell Lysate at 40 ug, A431 (huamn) Cell Lysate at 40 ug, RAW264.7 (Mouse) Cell Lysate at 40 ug, Primary: Anti-Collagen I (orb312178) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 110 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated (orb312178) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.