Collagen I Mouse Monoclonal Antibody

• Catalog Number: orb2563523
• Category: Antibodies
• Description: Collagen I Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3C2
• Tested applications: IF, IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen I
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:200-500, IHC-F=1:200-500, IF=1:200-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 139 kDa
• Target: COL1A1
• UniProt ID: P02452
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Collagen type I; Alpha 1 type I collagen; Alpha 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Human Uterus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Prostate, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.