Collagen alpha-1 (I) chain Rabbit Polyclonal Antibody

• Catalog Number: orb500860
• Category: Antibodies
• Description: Collagen alpha-1 (I) chain Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Human, Mouse
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen alpha-1(I) chain (1051-1218aa)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 130 kDa
• Target: COL1A1
• UniProt ID: P02452
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: Collagen type I; Alpha 1 type I collagen; COL1A1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Collagen alpha-1 (I) chain) Polyclonal Antibody, Unconjugated (orb500860) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.