COL2A1 antibody

• Catalog Number: orb10436
• Category: Antibodies
• Description: COL2A1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human COL2A1. Please contact us for the exact immunogen sequence. The peptide is available as orb374716.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: WB:1:200, IF/ICC: 1:300, IHC-P:1:300 (based on 0.5 mg/ml)
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 117 kDa
• Target: COL2A1
• Entrez: 1280
• UniProt ID: P02458, P05539, P28481
• NCBI: 31, 001835, 41, 001844
• RRID: AB_10753183
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-CB11 antibody, anti-COL11A3 antibody, anti-Dm
• Note: For research use only
• Application notes: A customer has successfully used this antibody in IHC
• Expiration Date: 12 months from date of receipt.

References

Kuo, Chang-Yi et al. Incorporation of chitosan in biomimetic gelatin/chondroitin-6-sulfate/hyaluronan cryogel for cartilage tissue engineering Carbohydr Polym, 117, 722-730 (2015)

PubMed: 25498693

IHC-P staining of pig liver tissue using Collagen II antibody (dilution at 1:300)

IF analysis of rabbit meniscus tissue using Collagen II antibody (dilution of primary antibody at 1:300)

Immunohistochemical analysis of formalin fixed and paraffin embedded rat intervertebral disc using Collagen II antibody (primary antibody at 1:300)

Immunohistochemical analysis of paraffin-embedded collagen of rat knee joint using Collagen II antibody (dilution of primary antibody - 1:300)

IHC-P of rat knee joint collagen (Collagen II antibody at 1:200)

Western blot analysis of human breast cancer (Lane 1), breast cancer (Lane 2) using Collagen II antibody (primary antibody at 1:200)

IHC-P of human esophageal carcinoma (Collagen II antibody atb 1:300)

Immunohistochemical staining of paraffin embedded human thyroid carcinoma tissue using Collagen II antibody (primary antibody at 1:300)

WB analysis of human breast cancer (Lane 1), breast cancer (Lane 2) using Collagen II antibody (dilution of primary antibody - 1:100)

Immunofluorescence analysis of mouse brain tissue using anti-Collagen II (dilution of primary antibody - 1:300)

IF image of mouse lung tissue using anti-Collagen II (primary antibody at 1:300)

IF analysis of mouse lung tissue using Collagen II antibody (dilution of primary antibody at 1:300)

Immunohistochemical staining of mouse lung tissue using anti-Collagen II (dilution of primary antibody - 1:300)

Immunofluorescence image of mouse brain tissue using anti-Collagen II (dilution at 1:300)