COL1A1 antibody

• Catalog Number: orb345844
• Category: Antibodies
• Description: COL1A1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, IP, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Collagen Type I from human and bovine placenta.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:3,000 - 1:6,000, FC: 5µg/mL, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:3,000 - 1:6,000
• Form/Appearance: Lyophilized
• Purity: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
• Conjugation: HRP
• UniProt ID: P02452
• NCBI: NP_000079.2
• Storage: Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Buffer/Preservatives: 0.01% (w/v) Thimerosal
• Alternative names: rabbit anti-collagen type I antibody peroxidase co
• Note: For research use only
• Application notes: Anti-COLLAGEN Type I Peroxidase Conjugated Antibody is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry of Anti-Collagen Type I Biotin Conjugated Antibody (orb345868). Cells: mouse lung. Stimulation: none. Primary antibody: biotin conjugated anti-collagen type I antibody. Secondary antibody: PE-conjugated CD45 and PE-conjugated anti-collagen type I secondary antibody.

Flow Cytometry of Rabbit Anti-Collagen 1 Antibody. Cells: primary adult human dermal fibroblast cells. Stimulation: none. Primary antibody: Biotin-Conjugated Collagen 1 antibody (orb345868) at 5 µg/mL for 45 min at 4°C. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1:500 for 15 min at RT.

Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin at pH6. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody. Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

Western Blot of Rabbit anti-Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 µg per lane. Protein staining shown below western blot depicts equal protein loading. Primary antibody: Anti-Collagen I antibody at 0.2–2 µg/10 ml for overnight at 4°C. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 µg/10 ml for overnight at 4°C. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band(s): none.