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Catalog Number | orb1294293 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to COL1A1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
Dilution range | Western Blot 1:1000-1:1500 Immunofluorescence 1:300–1:500 Immunohistochemistry (Paraffin) 1:100–1:300 |
Conjugation | Unconjugated |
Target | COL1A1 / Collagen I |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
COL1A1/Collagen I antibody - at 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min. Cells were stained with orb1294293 anti-COL1A1/Collagen I antibody (red) at 1:400 and 4°C.
Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1/Collagen I with orb1294293 at 1/150.
Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1/Collagen I with orb1294293 at 1/150.
Anti-COL1A1/Collagen I antibody - at 1/1000 dilution. Lysates at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1/Collagen I with orb1294293 at 1/100.
Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1/Collagen I with orb1294293 at 1/100.
COL1A1/Collagen I antibody - at 1/250 dilution. Lysates at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.
IHC-P, WB | |
Bovine, Equine, Monkey, Sheep | |
Canine, Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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