CNTF Antibody

• Catalog Number: orb196238
• Category: Antibodies
• Description: CNTF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived rat CNTF recombinant protein (Position: A2-M200). Rat CNTF shares 84% and 95% amino acid (aa) sequences identity with human and mouse CNTF, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22854 MW
• UniProt ID: P20294
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Ciliary neurotrophic factor;CNTF;Cntf;
• Note: For research use only
• Application notes: WB: The detection limit for CNTF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CNTF using anti-CNTF antibody.Lane 1:rat PC-12 cell; 2:rat NRK cell; 3:rat RH-35 cell; 4:rat brain tissue; 5:rat liver tissue; 6:rat spleen tissue; 7:rat testis tissue.

IHC analysis of CNTF using anti-CNTF antibody. CNTF was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of CNTF using anti-CNTF antibody. CNTF was detected in a paraffin-embedded section of rat brain tissue.