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Item 1 of 4
Item 1 of 4
CNPase Recombinant Mouse Monoclonal Antibody
Catalog Number: orb1152051
| Catalog Number | orb1152051 |
|---|---|
| Category | Antibodies |
| Description | CNPase Recombinant Mouse Monoclonal Antibody |
| Species/Host | Mouse |
| Clonality | Recombinant |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Rat |
| Reactivity | Human, Rat |
| Isotype | IgG1/Kappa |
| Immunogen | KLH conjugated synthetic peptide derived from human CNPase |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:200-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:20-50, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 48 kDa |
| Target | CNP |
| UniProt ID | P09543 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | 2', 3'-cyclic-nucleotide 3'-phosphodiesterase; CNP Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Lysate: 1: HeLa, 2: HEK-293, 3: Rat cerebellum, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 48 kDa, Observed MW: 48 kDa.
Cell line: SH-SY5Y, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152051.
Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Primary: Anti-CNPase (orb1152051) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 46 kDa.
SHSY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CNPase) polyclonal Antibody, Unconjugated (orb1152051) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
