CLU/Clusterin Antibody

• Catalog Number: orb1537927
• Category: Antibodies
• Description: CLU/Clusterin Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1A11
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1,k
• Immunogen: Recombinant human Clusterin (1-333aa) purified from E. coli.
• Concentration: 1 mg/ml
• Dilution range: ELISA, IHC, IHC-P (10 µg/ml), WB (1:1000 - 1:2000)
• Conjugation: Unconjugated
• Target: CLU / Clusterin
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4, 0.1% Sodium Azide. PBS, pH 7.4, 0.1% Sodium Azide
• Alternative names: CLU, APO-J, Apolipoprotein J, CLU1, Clusterin, Com
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B2937 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2937 was determined to be 10 ug/ml.
• Expiration Date: 12 months from date of receipt.

Cell lysates of U87 MG (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.