CLU antibody

• Catalog Number: orb538946
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CLU
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 314-449 of human Clusterin alpha chain (NP_001822.3).
• Dilution range: WB: 1:500-1:2000
• Conjugation: Unconjugated
• MW: 37-40kDa
• UniProt ID: P10909
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: CLU, AAG4, APO-J, APOJ, CLI, CLU1, CLU2, KUB1, NA1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Clusterin alpha chain Rabbit pAb (orb538946) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human esophageal using Clusterin alpha chain Rabbit pAb (orb538946) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human lung using Clusterin alpha chain Rabbit pAb (orb538946) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat spleen using Clusterin alpha chain Rabbit pAb (orb538946) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western blot analysis of various lysates using Clusterin alpha chain Rabbit pAb (orb538946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.