CLU antibody

• Catalog Number: orb536452
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CLU
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-449 of human Clusterin alpha chain (NP_976084.1).
• Dilution range: IF: 1:50-1:200
• Conjugation: Unconjugated
• MW: 38kDa/57kDa
• UniProt ID: P10909
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Alternative names: CLU, AAG4, APO-J, APOJ, CLI, CLU1, CLU2, KUB1, NA1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of U2OS cells using Clusterin Rabbit pAb (orb536452). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Clusterin Rabbit pAb (orb536452) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Clusterin Rabbit pAb (orb536452) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Western blot analysis of various lysates using Clusterin Rabbit pAb (orb536452) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.