You have no items in your shopping cart.
Cart summary
Item 1 of 3
Item 1 of 3
Cleaved LC3B Antibody
Catalog Number: orb1933516
| Catalog Number | orb1933516 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB15839;RB28608 |
| Tested applications | ICC, IF |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:100, IF: 1:10~50, ICC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 14688 Da |
| Target | This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B. |
| UniProt ID | Q9GZQ8 |
| NCBI | NP_073729.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Microtubule-associated proteins 1A/1B light chain Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with antibody at dilution 1:100.
Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures.
Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 μM, 16 h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
