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Catalog Number | orb1259694 |
---|---|
Category | Antibodies |
Description | CLCA1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 22-150 of human CLCA1 (NP_001276.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 85kDa |
Target | CLCA1 |
UniProt ID | A8K7I4 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | CLCA1, chloride channel, calcium activated, family Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CLCA1 antibody (orb1259694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Immunofluorescence analysis of rat colon using CLCA1 Polyclonal Antibody (orb1259694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human colon carcinoma using CLCA1 Polyclonal Antibody (orb1259694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse colon using CLCA1 Polyclonal Antibody (orb1259694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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