CKB Antibody / Creatine kinase B

• Catalog Number: orb1825474
• Category: Antibodies
• Description: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CKBB/6567
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human full-length protein was used as the immunogen for the Creatine kinase B antibody.
• Dilution range: ELISA (Order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Creatine kinase B antibody is available for research use only.
• UniProt ID: P12277
• Storage: Aliquot the Creatine kinase B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HEK293 cell lysate with Creatine kinase B antibody (clone CKBB/6567). Predicted molecular weight ~43 kDa.

Western blot testing of human Y79 cell lysate with Creatine kinase B antibody (clone CKBB/6567). Predicted molecular weight ~43 kDa.

IHC staining of FFPE human salivary gland tissue with Creatine kinase B antibody (clone CKBB/6567). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human brain tissue with Creatine kinase B antibody (clone CKBB/6567). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.