CK1 alpha (phospho-Y321) antibody

• Catalog Number: orb213787
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CSNK1A1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y321 of human CK1 alpha protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CSNK1A1
• Entrez: 93687, 113927, 1452
• UniProt ID: Q8BK63, P97633, P48729
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti CKI-alpha antibody, anti CK1 antibody, anti C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CK1 alpha (Phospho-Y321) expression in HEK293T UV-treated (A), Jurkat (B), K562 (C), mouse liver (D), H9C2 UV-treated (E) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 45 kD)

Immunohistochemical analysis of CK1 alpha (Phospho-Y321) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.