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Catalog Number | orb1256933 |
---|---|
Category | Antibodies |
Description | CIRBP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (NP_001271.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:10 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 18kDa |
Target | CIRBP |
UniProt ID | Q14011 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | CIRBP, CIRP Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:10 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CIRBP antibody (orb1256933) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CIRBP antibody (orb1256933) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CIRBP antibody (orb1256933). Blue: DAPI for nuclear staining.
ELISA, FC, IF, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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