Chromogranin A Antibody / CHGA

• Catalog Number: orb699498
• Category: Antibodies
• Description: Chromogranin A (CGA) is an acidic protein which is reported to be widely expressed in neural tissues and in secretory granules of human endocrine cells e.g. parathyroid gland, adrenal medulla, anterior pituitary gland, islet cells of the pancreas and C cells of the thyroid. GA is found in the large majority of epithelial NE tumors: pituitary adenomas, parathyroid and pancreatic islet cell tumors, medullary thyroid carcinoma, and carcinoid tumors. Together with synaptophysin, CGA is the most important marker for the identification of NE and neuronal tumors and NE/neuronal differentiation in other tumors.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CHGA/4544R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 400-600 from the human protein was used as the immunogen for the recombinant Chromogranin A antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Chromogranin A antibody is available for research use only.
• UniProt ID: P10645
• Storage: Store the recombinant Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Chromogranin A antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human pancreas with recombinant Chromogranin A antibody (clone CHGA/4544R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human pancreas with recombinant Chromogranin A antibody (clone CHGA/4544R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.