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Catalog Number | orb235096 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CHK2 (phospho-T383) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Porcine, Rat |
Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T383 of human CHK2 protein. The exact sequence is proprietary. |
Dilution range | WB: 1:500-1000, IF/ICC: 1:100-500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | CHEK2 |
Entrez | 50883, 11200 |
UniProt ID | O96017, Q9Z265 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti CDS1 antibody, anti CHK2 antibody, anti RAD53 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CHK2 (Phospho-T383) expression in HEK293T (A), HEK293T-UV-30 min (B) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 62 kD)
Immunofluorescent analysis of CHK2 (Phospho-T383) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ELISA, IF, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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