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Catalog Number | orb1258335 |
---|---|
Category | Antibodies |
Description | CHIT1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 217-466 of human CHIT1 (NP_003456.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 52kDa |
Target | CHIT1 |
UniProt ID | Q13231 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | CHIT1, plasma methylumbelliferyl tetra-N-acetylchi Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CHIT1 antibody (orb1258335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human normal stomach using CHIT1 antibody (orb1258335) at dilution of 1:200 (40x lens).
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