Chicken IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb346890
• Category: Antibodies
• Description: Chicken IgG (H&L) antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in goat.
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:10,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Chicken IgG and Chicken Serum.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Anti-Chicken Antibody, Gt-a-Chicken IgG, Anti-Chic
• Note: For research use only
• Application notes: Anti-Chicken IgG (H&L) HRP Conjugated Antibody is tested in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Anti-Chicken IgG HRP Antibody is suitable for IHC. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

ELISA results of Goat Anti-Chicken IgG Antibody Peroxidase Conjugated tested against purified Chicken IgG protein. Each well was coated in duplicate with 1.0 µg of Chicken IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:97900. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.

Western Blot of Goat Anti-Chicken IgG Peroxidase Conjugated Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: RFP (p/n orb345960) [0.5 µg]. Lane 3: RFP (p/n orb345960) [0.1 µg]. Lane 4: HEK293T whole cell lysate spiked RFP [10 µg+ 0.1 µg]. Primary Antibody: Rabbit Anti-RFP (p/n orb345391) at 1.0 µg/mL o/n at 4°C. Secondary Antibody: Goat Anti-Chicken IgG HRP (p/n orb346890) at 1:40000 for 30 mins at RT. Blocking: Universal BlockOut Buffer (p/n orb348644) for 30 mins at RT. Predicted MW: ~27 kDa.