Chicken IgG (H&L) antibody

• Catalog Number: orb346905
• Category: Antibodies
• Description: Chicken IgG (H&L) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in rabbit.
• Concentration: 10 mg/mL
• Dilution range: ELISA: 1:200,000 - 1:400,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:30,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit anti-Chicken IgG Antibody, Chicken Secondar
• Note: For research use only
• Application notes: Anti-Chicken IgG (H&L) has been tested by ELISA, SDS-PAGE, and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Chicken IgG Antibody. Lane 1: Chicken IgG. Load: 100 ng per lane. Primary antibody: Chicken IgG antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit anti-Chicken secondary antibody at 1:20000 for 30 min at RT. Block: 5% BLOTTO for 30 min at RT. Predicted/Observed size: 72 and 28 kDa, 72 and 28 kDa for Chicken IgY.

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n orb346905). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG Fab Fragment. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n orb346905) 1:3000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.