Chicken IgG (H&L) antibody (FITC)

• Catalog Number: orb346927
• Category: Antibodies
• Description: Chicken IgG (H&L) antibody (FITC)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Chicken IgG whole molecule
• Concentration: 1 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Donkey Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Donkey anti-Chicken IgG Antibody Fluorescein Conju
• Note: For research use only
• Application notes: Anti-Chicken IgG Fluorescein Antibody has been tested by dot blot and western blot. This product is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Expiration Date: 12 months from date of receipt.

Western Blot of Fluorescein conjugated Donkey anti-Chicken IgG Pre-adsorbed secondary antibody. Lane 1: Chicken IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein donkey secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 72 kDa, 25 & 72 kDa for Chicken IgY.

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n orb346926). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG Fab Fragment. Lane 4: Chicken IgM Whole Molecule (p/n orb346054). Lane 5: Chicken Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n orb346926) 1:3000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347994) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.