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Catalog Number | orb346919 |
---|---|
Category | Antibodies |
Description | Chicken IgG (H&L) antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Gallus |
Isotype | IgG |
Immunogen | Anti-Chicken IgY / IgG whole molecule was produced by repeated immunization with Chicken IgY / IgG whole molecule in rabbit. |
Concentration | 1.5 mg/mL |
Dilution range | ELISA: 1:500,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000 |
Form/Appearance | Lyophilized |
Purity | Biotin Conjugated Anti-Chicken IgG / IgY secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG / IgY coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Chicken IgG / IgY. Some light chain cross reactivity may be observed against chicken immunoglobulins. |
Conjugation | Biotin |
Storage | Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit anti-Chicken IgG Biotin Conjugated Antibody Read more... |
Note | For research use only |
Application notes | Secondary antibodies are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods |
Expiration Date | 12 months from date of receipt. |
Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n orb346905). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG Fab Fragment. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n orb346905) 1:3000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.
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