Chicken IgG (H&L) antibody (Alkaline Phosphatase)

• Catalog Number: orb346916
• Category: Antibodies
• Description: Chicken IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Chicken IgY / IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Chicken IgY / IgG Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY / IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, and Chicken IgY / IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
• Conjugation: AP
• Storage: Store Secondary Antibody at 4° C before opening. DO NOT FREEZE. This antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: Rabbit anti-Chicken IgG Alkaline Phosphatase Conju
• Note: For research use only
• Application notes: Anti-Chicken IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n orb346905). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG Fab Fragment. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n orb346905) 1:3000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.