Chicken IgG F(c) antibody (Alkaline Phosphatase)

• Catalog Number: orb346917
• Category: Antibodies
• Description: Chicken IgG F(c) antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Chicken IgG F(c) fragment
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(c) and Chicken Serum. No reaction was observed against Chicken IgG F(ab).
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: rabbit anti-Chicken IgG F(c) Antibody Alkaline Pho
• Note: For research use only
• Application notes: Anti-Chicken IgG F(c) Antibody Alkaline Phosphatase Conjugated has been tested by ELISA. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:9,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Unconjugated Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n orb346906). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG Fab Fragment. Lane 4: Chicken IgM Whole Molecule (p/n orb346054). Lane 5: Chicken Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n orb346906) 1:2000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.