Chicken IgG F(ab')2 Peroxidase Antibody

• Catalog Number: orb346061
• Category: Proteins
• Description: Chicken IgG F(ab')2 Peroxidase Antibody
• Tested applications: SDS-PAGE
• Isotype: IgG F(ab')2
• Concentration: 1.0 mg/mL
• Form/Appearance: Lyophilized
• Purity: This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken IgG, anti-Chicken IgG F(ab’)2 and anti-Chicken Serum. No reaction was observed against anti-Chicken IgG F(c) or anti-Pepsin.
• Conjugation: HRP
• Source: Chicken
• Biological Origin: Chicken
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Chicken IgG F(ab')2 fragment peroxidase conjugated
• Note: For research use only
• Application notes: Chicken IgG F(ab')2 fragment Peroxidase conjugated has been tested by SDS-PAGE and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Reduced Chicken IgG Fab Fragment (p/n orb2652771). Lane 4: Reduced Chicken IgM Whole Molecule (p/n orb346054). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.