Chicken IgG F(ab')2 antibody (Alkaline Phosphatase)

• Catalog Number: orb346918
• Category: Antibodies
• Description: Chicken IgG F(ab')2 antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Chicken IgG F(ab')2 fragment
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:3,500, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab’)2 and Chicken Serum. No reaction was observed against Chicken IgG F(c).
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Chicken IgG F(ab')2 Antibody Alkaline
• Note: For research use only
• Application notes: Anti-Chicken IgG F(ab')2 Alkaline Phosphatase antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rabbit anti-Chicken IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated. Antigen: Chicken IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Chicken IgG/IgY F(ab')2 Antibody Alkaline Phosphatase Conjugated at 1:1000 for 1 hr at RT. Block: orb348637 for 1 hr at RT.

Western Blot of Unconjugated Anti-Chicken IgG F(ab')2 (RABBIT) Antibody (p/n orb346907). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG F(ab) Fragment. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(ab')2 (RABBIT) Antibody (p/n orb346907) 1:5000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.