Chicken IgG Antibody

• Catalog Number: orb346050
• Category: Proteins
• Description: Chicken IgG Antibody
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Isotype: IgG
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Chicken IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed extensive dialysis against the buffer stated above. Chicken IgG whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG and anti-Chicken Serum.
• Conjugation: Unconjugated
• Source: Chicken
• Biological Origin: Chicken
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Chicken immunoglobulin G, Chicken IgG, Chicken IgY
• Note: For research use only
• Application notes: Chicken IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS PAGE of Chicken IgG/IgY Whole Molecule. Lane 1: Non-Reduced Chicken IgG Whole Molecule. Lane 2: 5 µL Opal Prestained Marker. Lane 3: Reduced Chicken IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-Reduced at 160 kDa/Observed at 245 kDa; Reduced at 72, 25 kDa. Non-reduced IgG migrates slightly higher.

SDS-PAGE of Chicken IgG/IgY Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane M: 5 µL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule Rhodamine Conjugated (p/n orb346044). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule (p/n orb346054). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.