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Catalog Number | orb1258748 |
---|---|
Category | Antibodies |
Description | CHI3L1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 22-240 of human YKL-40 / CHI3L1 (NP_001267.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 43kDa |
Target | CHI3L1 |
UniProt ID | P36222 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | CHI3L1, DKFZp686N19119, FLJ38139, GP39, HC-gp39, H Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using YKL-40/CHI3L1 antibody (orb1258748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat spleen using YKL-40/CHI3L1 antibody (orb1258748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using YKL-40/CHI3L1 antibody (orb1258748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using YKL-40/CHI3L1 antibody (orb1258748) at dilution of 1:100 (40x lens).
IF, WB | |
Bovine, Canine, Goat, Porcine, Rabbit, Rat | |
Human, Monkey | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ICC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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