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CHGA Rabbit Polyclonal Antibody

Catalog Number: orb2563460

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb2563460
CategoryAntibodies
DescriptionCHGA Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityHuman, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenRecombinant mouse CHGA protein (272-463/463aa)
Concentration1mg/ml
Dilution rangeIHC-P=1:500-1000, WB=1:500-2000, Flow-Cyt=2ug/Test, IF=1:500-1000, IHC-F=1:500-1000
Form/AppearanceLiquid
ConjugationUnconjugated
MW48 kDa
TargetCHGA
UniProt IDP26339
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesChromogranin-A; Chromogranin A; beta Granin; CGA;
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NoteFor research use only
Expiration Date12 months from date of receipt.
CHGA Rabbit Polyclonal Antibody

Blank control (black line): SH-SY5Y. Primary Antibody (green line): Rabbit Anti-CHGA antibody (orb2563460), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human Gastric fundus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:800overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:1000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:600 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:600overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CHGA) Polyclonal Antibody, Unconjugated (orb2563460) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Duodenum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CHGA Polyclonal Antibody, Unconjugated (orb2563460) at 1:600 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, orb868605), DAPI (blue) was used to stain the cell nuclei.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CHGA Polyclonal Antibody, Unconjugated (orb2563460) at 1:600 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, orb868605), DAPI (blue) was used to stain the cell nuclei.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CHGA Polyclonal Antibody, Unconjugated (orb2563460) at 1:600 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, orb868605), DAPI (blue) was used to stain the cell nuclei.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CHGA Polyclonal Antibody, Unconjugated (orb2563460) at 1:600 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Purple, orb868605), DAPI (blue) was used to stain the cell nuclei.

CHGA Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Adrenal gland tissue lysates, Lane 2: Rat Adrenal gland tissue lysates, Lane 3: Recombinant mouse CHGA protein, C-His (HEK293) (orb1975913), Lane 4: Mouse NIH/3T3 cell lysates (negative control), Primary: Anti-CHGA (orb2563460) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 33, 76 kDa.

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