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CHGA Rabbit Polyclonal Antibody

Catalog Number: orb10377

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10377
CategoryAntibodies
DescriptionCHGA Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsICC, IF, IHC-Fr, IHC-P
Predicted ReactivityMouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human CHGA (421-470/457aa)
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW48 kDa
TargetCHGA
UniProt IDP10645
RRIDAB_10762618
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesChromogranin-A; Chromogranin A; beta Granin; CGA;
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NoteFor research use only
Expiration Date12 months from date of receipt.
CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (orb10377) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (orb10377) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (orb10377) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (orb10377) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (orb10377) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CHGA Rabbit Polyclonal Antibody

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Chromogranin A) polyclonal Antibody, Unconjugated (orb10377) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

CHGA Rabbit Polyclonal Antibody

Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CGA/Chromogranin A Polyclonal Antibody, Unconjugated (orb10377) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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