CHGA Antibody

• Catalog Number: orb1274273
• Category: Antibodies
• Description: CHGA Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CHGA/1773R
• Tested applications: FC, IF, IHC-P
• Reactivity: Human
• Isotype: IgG, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant Chromogranin A antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Chromogranin A antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CHGA
• UniProt ID: P10645
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Chromogranin-A, CgA, Pituitary secretory protein I
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Chromogranin A antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC analysis of FFPE human parathyroid gland stained with recombinant Chromogranin A antibody (clone CHGA/1773R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

IHC analysis of FFPE human pancreas stained with recombinant Chromogranin A antibody (clone CHGA/1773R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.