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Catalog Number | orb1261835 |
---|---|
Category | Antibodies |
Description | CHEK2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human CHEK2 (NP_009125.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 60kDa |
Target | CHEK2 |
UniProt ID | O96017 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Chk2 Antibody: CDS1, CHK2, LFS2, RAD53, hCds1, HuC Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CHEK2 antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using CHEK2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CHEK2 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using CHEK2 antibody.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug CHEK2 antibody. Western blot was performed from the immunoprecipitate using CHEK2 antibody (orb1261835) at a dilition of 1:1000.
FC, WB | |
Human, Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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