You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb213730 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CHEK1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S286 of human CHK1 protein. The exact sequence is proprietary. |
Dilution range | WB: 1-500-1-1000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | CHEK1 |
Entrez | 140583, 12649, 1111 |
UniProt ID | O35280, O14757, Q91ZN7 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti CHK1 antibody, anti Serine/threonine-protein Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CHK1 (Phospho-S286) expression in HEK293T (A), Jurkat (B) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 60 kD)
Immunofluorescent analysis of CHK1 (Phospho-S286) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
ELISA, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Canine, Equine, Gallus, Human | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Canine, Equine, Gallus, Human | |
Mouse | |
Rabbit | |
Polyclonal | |
Biotin |
Filter by Rating