CHD4 Antibody / Chromodomain helicase DNA binding protein 4

• Catalog Number: orb639584
• Category: Antibodies
• Description: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3F2/4
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2a, kappa
• Immunogen: Amino acids ASGLGSPSPCSAGSEEEDM and CSRLANRAPEPPPQQVAQQQ were used as the immunogen for the CHD4 antibody.
• Dilution range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CHD4 antibody is available for research use only.
• UniProt ID: Q14839
• Storage: Store the CHD4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of permeabilized human MCF7 cells with CHD4 antibody (clone 3F2/4, green) and Phalloidin (red).

IHC staining of FFPE human breast carcinoma with CHD4 antibody (clone 3F2/4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human SK-BR-3 cells with CHD4 antibody. Predicted molecular weight ~260 kDa.

SDS-PAGE analysis of purified, BSA-free CHD4 antibody (clone 3F2/4) as confirmation of integrity and purity.