CHAT Antibody

• Catalog Number: orb625745
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Chat
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: choline acetyltransferase
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:500
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 47-50, 66-70 kDa
• Target: CHAT
• UniProt ID: P28329
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IP Result of anti-CHAT (IP: orb625745, 4 ug; Detection: orb625745 1:500) with rat brain tissue lysate 4000 ug.

rat brain tissue were subjected to SDS PAGE followed by western blot with orb625745 (CHAT Antibody) at dilution of 1:600.

Immunohistochemistry of paraffin-embedded human brain tissue slide using orb625745 (CHAT Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).